CLINICAL APPLICATION OF CLOSTRIDIUM DIFFICILE TOXIN A/B DETECTION IN THE OUTSIDE HOSPITAL AND NOSOCOMIAL INFECTION IN TAISHAN AREA / 现代医院

Objective To investigate the application of Clostridium difficile toxin A/B detection on the out-side hospital and nosocomial infection in Taishan area .Methods The stool samples of 528 diarrhea patients newly admitted from May 2013 to October 2014 were collected as the outside hospital suspicious group , and the patients were aged 8 to 75 years old, including 249 males and 279 females.Relatively, the stool samples of 523 patients hos-pitalized more than a week and treated with antibiotics more than 3 days were collected as the nosocomial suspicious group, and the patients aged 7 to 74 years, 209 males and 314 females.Clostridium difficile toxin A/B was detected, and the comprehensive statistical analysis combined with thebacterial culture results , clinical diagnosis and curative effect was made .Results The clostridium difficile infection rate outside the hospital was 4% and the infection rate in hospital after using antibiotics was 16%.By Clostridium difficile toxin A/B detection , the positive rate of outside hospital infection was 85%, and that of nosocomial infection was 90%.Conclusion The Clostridium difficile toxin A/B detection is worthy of clinical application for its rapid and accurate outside hospital and nosocomial infection de -tection in Taishan area .

Evaluation of Rapid Assay (Tox A/B Quik Chek) for the Detection of Clostridium difficile Toxins A and B / 대한임상미생물학회지

BACKGROUND: Toxin immunoassay is widely used for rapid diagnosis of Clostridium difficile-associated diarrhea. The aim of this study was to evaluate the performance of Tox A/B Quik Chek test (TECHLAB, Blacksburg, VA, USA) compared to toxigenic culture. METHODS: From September 2006 to August 2007, 959 stools were examined by Tox A/B Quik Chek test and toxigenic culture (C. difficile culture plus tcdB PCR using colonies obtained from culture). RESULTS: Compared to the results of toxigenic culture, the sensitivity and specificity of Tox A/B Quik Chek test were 47.5% and 97.5%, respectively. CONCLUSION: The sensitivity of Tox A/B Quik Chek test was not high, but the specificity was high. Although Tox A/B Quik Chek test alone is not sufficient to diagnose Clostridium difficile-associated diarrhea, it may aid rapid diagnosis, early treatment and prevention of nosocomial spread of the infection, if supplemented by C. difficile culture or tissue culture cytotoxin assay.